. Author manuscript; available in PMC: 2009 Sep 17.

Published in final edited form as: Exp Biol Med (Maywood). 2008 Apr 29;233(7):849–859. doi: 10.3181/0712-RM-352

Table 2.

Interaction of the Long Cytoplasmic Domain of CEACAM1 with β-Cateninin a Quantitative Yeast Two-Hybrid Assay^a

Bait^b	Prey	Response^c	P-value^d
None	Arm-β-catenin	0.079 ± 0.001
Long cyto. domain	none	0.046 ± 0.001
Long cyto. domain	Arm-β-catenin	0.468 ± 0.015	0.001
1-60hTcf-4	Arm-β-catenin	0.206 ± 0.01	0.001
Long cyto. domain	FL-β-catenin	0.378 ± 0.011	0.001
1-60hTcf-4	FL-β-catenin	0.422 ± 0.02	0.001

See methods for the assay details.

Bait (e.g., the long cytoplasmic domain of CEACAM1) was fused to Gal4 BD (binding domain) and prey (e.g., the armadillo repeat (Arm) or full length (FL) β-catenin) was fused to the Gal4 AD (activation domain). 1-60hTcf-4 was used as a positive control.

A_410nm normalized for cell density.

Calculated by the chi-squared method.