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. Author manuscript; available in PMC: 2009 Sep 17.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Apr 29;233(7):849–859. doi: 10.3181/0712-RM-352

Table 3.

Binding Constants for the Interaction of Peptides from the Long Cytoplasmic Domain of CEACAM1 with GST-β-Catenina

Peptide kon (M −1sec−1) koff (sec −1) KD (nM)
Wild type 7.95 × 104 4.73 × 10−4 5.9
K470A 5.88 × 103 5.05 × 10−4 86
H469A 8.98 × 103 4.94 × 10−4 54
a

The GST-fusion protein (50 μg/ml) of the armadillo repeats (residues 136–671) of β-catenin was immobilized to a BIAcore CM5 chip and the binding of wild type or mutated version of the long cytoplasmic domain of CEACAM1 flowed over it vs. a CM5 chip immobilized with GST. All peptides were at 4 μM.