Table 4.

Binding of GST-Fusion Proteins to β-Catenin in Jurkat Cell Lysates^a

GST-fusion protein	β-catenin (normalized)
WT	1.00
D465A	0.48
E468A	0.78
H469A	0.25
K470A	0.46
P471A	0.88
S472A	0.49
D479A	0.93
Δ	0.88
Tcf-4	3.46
GST	0.06

^aGST fusion proteins containing either the wild type sequence (451–526) of the long cytoplasmic domain or selected mutants were incubated with lysates of Jurkat cells, washed with Profound lysis buffer in TBS, and bound proteins eluted with 100 mM reduced glutathione, run on SDS gels, and western blotted with anti- β-catenin and GST antibodies. The western blots were scanned and quantitated on the LiCor Odessey and β-catenin amounts were normalized first to GST-fusion protein and then to the wild type GST fusion protein. Alternatively, the input of GST-fusion protein was normalized to Coomassie Blue stained gels as shown in Figure 4. Δ, truncated version (451–484) of the long cytoplasmic domain; Tcf-4, full-length version of hTcf-4; GST, GST only control.