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. Author manuscript; available in PMC: 2009 Sep 17.
Published in final edited form as: Parasitol Res. 2007 Dec 6;102(3):437–446. doi: 10.1007/s00436-007-0784-4

Fig. 2.

Fig. 2

Expression, purification, and Western blot analysis of L. amazonensis PDI proteins. Recombinant plasmids containing the corresponding L. amazonensis PDI gene fragment (without the signal peptide) were transformed into BL21DE3 cells and induced for protein expression (1 mM IPTG for 4 h). Expressed PDI proteins were detected by electrophoresis in 12% SDS-PAGE and purified with His-tag columns (a). Western blots were performed for the detection of expressed PDI proteins by anti-His mAb (b) or pooled sera from L. amazonensis-infected mice (c). Lanes 1, 5, and 9 are for LaPDI15; lanes 2, 6, and 10 are for LaPDI40; lanes 3, 7, and 11 are for LaPDI47; and lanes 4, 8, and 12 are for LaPDI52. Arrows indicate the proteins with expected sizes