Fig. 4.

PDI activity of L. amazonensis promastigotes and the effects of PDI inhibitors in parasite growth, a Promastigote lysates were prepared from 1 × 10⁹ parasites and analyzed for PDI activity in the absence or presence of 2 mM bacitracin or 2 mM pCMBA. Results are presented as absorbance at OD₂₉₆, and representative results are shown from three repeated experiments, b L. amazonensis promastigotes (l × l0⁶/ml) were cultured in the absence or presence of indicated concentrations of PDI inhibitors for consecutive 5 days. Parasite numbers were counted microscopically, and results are presented as parasite numbers per ml (×10⁶). Shown are representative results from three independent repeats