. Author manuscript; available in PMC: 2009 Sep 17.

Published in final edited form as: Exp Cell Res. 2009 Mar 10;315(10):1668–1682. doi: 10.1016/j.yexcr.2009.02.026

Table 2.

Quantitation of vascular sprouting in the presenece or absence of VEGF or in Matrigel vs. growth factor reduced Matrigel.^a

Day	Day 4				Day 8				Day 11

Gel type	Regular		GF reduced		Regular		GF reduced		Regular		GF reduced

VEGF	+	−	+	−	+	−	+	−	+	−	+	−
No sprouts	31,25	50,49^*	27,26	50,48^**	25,26	31,38	27,24	22,21	23,22	21,25	16,11	18,14
Sprouts <250 µm	44,48	31,34^*	51,49	32,31^**	35,28	40,37	17,17	43,42^**	22,25	24,24	12,15^#	14,17^#
Sprouts >250 μm	25,27	19,17^*	22,25	18,21	40,46	29,25^*	56,59^#	36,38^**	55,53	55,51	72,74^##	68,69^#

EBs were grown in the regular Matrigel or growth factor reduced Matrigel in the presence or absence of 100 µg/ml VEGF. The numbers of sprouts were quantitated at days 4, 8, and 11 as follows: the number with no sprouts, the number with sprouts <250 µm, and the number with sprouts >250 µm. The experiment was repeated twice. Values were compared to the no VEGF treatment control (*, p<0.05,**, p<0.01) or regular vs. GF reduced Matrigel (#, p<0.05,##, p<0.01) by the Student's T test.