Table 4.
Quantitation of vascular sprouting inhibition by anti-PECAM1 antibody.a
Day | Day 4 | Day 8 | Day 11 | ||||||
---|---|---|---|---|---|---|---|---|---|
Treatment | No tx | Iso tx | PE tx | No tx | Iso tx | PE tx | No tx | Iso tx | PE tx |
No sprouts | 38,39 | 35,32 | 37,31 | 27,28 | 29,28 | 38,34* | 26,28 | 19,23 | 28,29 |
Sprouts <250 µm | 51,48 | 53,57 | 55,51 | 38,39 | 41,39 | 42,45 | 29,28 | 33,27 | 23,28 |
Sprouts >250 µm | 10,14 | 12,11 | 14,12 | 35,33 | 30,33 | 20,21** | 45,44 | 48,50 | 47,43 |
EBs were treated with 0 (No tx), 10 µg/ml of an isotype control Ab (Iso tx) or 10 µg/ml of anti-PEACAM1 antibody (PE tx) and the number of sprouts quantitated at days 4, 8, and 12 as follows: the number with no sprouts, the number with sprouts <250 µm, and the number with sprouts >250 µm. The experiment was repeated twice. All values were compared to the no treatment control by Student's T test (*, p<0.05,**, p<0.01).