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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Pept Sci. 2009 Feb;15(2):78–88. doi: 10.1002/psc.1096

Figure 6. Western blots demonstrating sensitivity and specificity of antisera towards the tetrameric rat GH-BP263-279 MAP dendrimer.

Figure 6

Samples of purified recombinant mouse GH-BP (100 ng) in Lane 1, rat serum (1 μL) in Lane 2 and rat liver extract (100 μg) in Lane 3 were loaded onto 10 % polyacrylamide gels and transferred onto nitrocellulose membranes. Blots were subsequently immunoprobed with rabbit anti-termeric rat GH-BP263-279 dendrimer antisera (BETO-8041) at dilutions of 10-2 (Panel A), 10-3 (Panel B) and 10-4 (Panel C). Antigen-antibody complexes were visualized by overlaying the blots with a horseradish perxidase-coupled goat anti-rabbit IgG and thereafter using a substrate (Supersignal™ West Dura Extended Duration HRP) that is enzymatically converted into a chemiluminescent product. Images of the chemiluminescent blots were acquired with a Kodak Image Station 2000R.