Figure 1.
C2C12 myoblasts (A) and myotubes (B) containing NF-κB response elements coupled to a luciferase reporter were plated for growth in triplicate groups and on the following day the media was changed to include ten different drugs at eleven different ½ log step increment doses. Twenty-four hours afterwards, 10ng/ml of TNF-α was added directly to the media and then 24 hours later, luciferase activity was recorded. The mean value for each parameter is shown, as well as the standard error amongst replicates.