Figure 5.

Respiratory function of newborn GLS1 mutants. A, Plethysmographic recordings showing baseline respiration in a wild-type (+/+) and a null mutant (−/−) mouse breathing room air (a, c) and ventilatory responses (b, d) to a hypercapneic challenge (6% CO₂). Vertical calibrations: 10 μl, inspiration up. B, Minute ventilation (V_E) in wild-type, heterozygous, and null mutant mice inhaling room air or 6% CO₂. ∗p < 0.05; ∗∗p < 0.01 versus wild-type mice; °°p < 0.01 versus baseline air breathing. GLS1−/− pups hypoventilated and showed a paradoxical hypercapneic response. Error bars represent SEM.