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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Dev Biol. 2009 Mar 31;330(1):131–141. doi: 10.1016/j.ydbio.2009.03.021

Figure 8.

Figure 8

Overexpression of Noggin to the palatal mesenchyme down-regulates the expression of Hand2 and Shh. (A) An E13.5 wild type control embryo shows undetectable fluorescent signals. (B) An E13.5 Osr2-Cre;pMes-Nog embryo shows EGFP signal in the craniofacial region (arrow) and forelimb FL) and hindlimb (HL). (C) An E13.5 wild type control shows barely detectable Noggin transcripts in the developing palatal shelf. Note Noggin expression in the tongue. (D) Strong Noggin expression (arrow) is detected in the palatal mesenchyme of an E13.5 Osr2-Cre;pMes-Nog embryo. (E, G, I) The expression of Hand2 in the anterior palate (E) and the epithelial junction of posterior palate (G) and Shh expression (I) in the MEE are detected in E13.5 controlled palatal shelves. (F, H, J) The expression of Hand2 is down-regulated in the anterior palate (F) and the epithelial junction of posterior palate (H) of E13.5 Osr2-Cre;pMes-Nog embryo. Shh expression is also down-regulated in the MEE of E13.5 Osr2-Cre;pMes-Nog palate (J). Note unaltered strong Hand2 expression in the tongue in (F). Arrows in (F, G, H) point to the MEE, and in (G, H) point to the epithelial junction of posterior palate and mandible.