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. Author manuscript; available in PMC: 2009 Sep 17.
Published in final edited form as: Nature. 2008 Aug 21;454(7207):1000–1004. doi: 10.1038/nature07221

Figure 1. BMP-7 induces brown, but not white, preadipocyte differentiation and the essential role of p38 MAPK in BMP-7-induced thermogenesis.

Figure 1

a, Oil Red O staining of brown preadipocytes and 3T3-L1 white preadipocytes grown in growth medium supplemented with BMPs or vehicle (control) for 8 days. b, Quantitative-RT-PCR (Q-RT-PCR) analysis for UCP-1 and Runx2 in brown preadipocytes treated with vehicle or BMPs in combination of insulin and T3 for 7 days. c, Q-RT-PCR analysis for PGC-1α and UCP-1 in response to 4 hrs of cAMP stimulation in brown preadipocytes differentiated in growth medium supplemented with BMP-7. Data are presented as mean ± SEM (n = 3). Asterisks depict statistically significant differences between control and experimental groups (* P < 0.05, ** P < 0.01, *** P < 0.001). d, Western blot analysis of phosphorylation of Smad1/5/8, p38 MAPK, and ATF-2 in response to 0, 10, and 30 min of BMP-7 stimulation in brown and white preadipocytes. e, Western blot analysis of UCP-1 in brown preadipocytes cultured in growth medium supplemented with vehicle or BMP-7 for 10 days. Three p38 MAPK inhibitors or vehicle (DMSO) were added to the cells 7 hrs prior to and throughout BMP-7 treatment. Cyclophilin A (Cyp A) serves as a loading control.