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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Bone. 2009 May 3;45(2):254–266. doi: 10.1016/j.bone.2009.04.239

Fig. 4. Affect of Exogenous Addition of BMP2 on BMP2 shRNA Treated Culture.

Fig. 4

All data is presented as the fold of the control untreated MSC cultures at 21 days. A) The exogenous addition of BMP2 protein rescue of nodule and mineral deposition in BMP2 shRNA inhibited MSCs cultures. Left panel shows the fold change in BMP2 mRNA expression in response to both BMP2 shRNA treated and BMP2 shRNA in the presence of exogenous addition of BMP2. Right panel shows the total mineral accumulation as measured by alizarin dye uptake by BMP2 rescue in comparison of NT shRNA and NT shRNA with BMP2 protein. B) Affect of Exogenous Addition of BMP2 in BMP2 shRNA Treated MSCs on the Expression of Various Transcription Factors that Control Skeletal Stem Cell Lineage Progression. Error bars represent standard deviation from replicate measurements from three separate cell preparations. P-value reflects the comparison of different treatment groups calculated by t-test and denoted as * p<0.05, ** p<0.01, *** p<0.001.