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. Author manuscript; available in PMC: 2009 Sep 17.
Published in final edited form as: Development. 2008 May;135(10):1887–1895. doi: 10.1242/dev.016147

Fig. 3. AV cushion formation is normal in Shh−/− mutant embryos.

Fig. 3

Wild-type (A) and Shh−/− (B) E10.5 AV cushion sagittal sections stained with PECAM1 (blue) and nuclear marker (green) demonstrated no significant change in morphology or total mesenchyme per cushion (quantitated in C; wild type and Shh−/− n=4). Identical analysis at E11.5 found no significant change in total mesenchyme cells or cushion volume (D; wild type and Shh−/− n=3). A, atria; V, ventricle; IAVC, inferior atrioventricular cushion; SAVC, superior atrioventricular cushion.