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. Author manuscript; available in PMC: 2010 Apr 10.
Published in final edited form as: J Chromatogr A. 2009 Feb 21;1216(15):3280–3289. doi: 10.1016/j.chroma.2009.02.030

Figure 2.

Figure 2

Figure 2

Figure 2

Typical LC-MS/MS chromatograms of targeted metabolites for extracts from M. extorquens AM1 grown on the ethylamine. (A) MRM-based measurement of the indicated seven metabolites and I.S. 13C10, 15N5-labeled ATP detected in positive ion mode. (B) Analogous data from negative mode. PEP, phosphoenolpyruvate; 3OHB, 3-hydroxybutyric acid. (C) Acyl-CoAs measurement in positive ion mode. 13C2-Labeled acetyl-CoA was the I.S. OHB-CoA, 3-hydroxybutyryl-CoA. Compounds labeled X and Y eluted at 6.75 min and 8.31 min and likely were methylsuccinyl-CoA and ethylmalonyl-CoA.