Table 3. List of metabolite concentrations determined by LC-MS/MS.
The absolute concentration of metabolites in M. extorquens AM1 cell extracts in picomole per gram of cell. Re/s is the ratio of intracellular metabolite concentrations in ethylamine and succinate cultures. The bold metabolites denote a significant difference (p < 0.05) between ethylamine and succinate cultures.
| Metabolites | Succinate (μmol/g) | Ethylamine (μmol/g) | Re/s |
|---|---|---|---|
| ATP | 2.84 ± 0.61 | 2.22 ± 0.45 | 0.78 ± 0.23 |
| ADP | 1.53 ± 0.37 | 1.89 ± 0.35 | 1.23 ± 0.38 |
| AMP | 0.27 ± 0.06 | 0.51 ± 0.08 | 1.89 ± 0.51 |
| NAD | 1.51 ± 0.36 | 2.00 ± 0.36 | 1.32 ± 0.40 |
| NADP | 0.36 ± 0.08 | 0.49 ± 0.08 | 1.36 ± 0.37 |
| NADH | 1.26 ± 0.35 | 1.22 ± 0.30 | 0.97 ± 0.36 |
| NADPH | 1.21 ± 0.40 | 0.84 ± 0.21 | 0.69 ± 0.29 |
| Glyine | 0.28 ± 0.05 | 0.23 ±0.03 | 0.82 ± 0.18 |
| Serine | 0.19 ± 0.04 | 0.27 ± 0.04 | 1.42 ± 0.36 |
| Pyruvate | 1.51 ± 0.23 | 0.50 ± 0.08 | 0.33 ± 0.07 |
| Fumaric acid | 0.69 ± 0.15 | 0.15 ± 0.02 | 0.22 ± 0.05 |
| Malic acid | 1.33 ± 0.30 | 0.37 ± 0.06 | 0.28 ± 0.08 |
| Phosphoenolpyruvate | 0.75 ± 0.13 | 0.25 ± 0.04 | 0.33 ± 0.08 |
| 3-Hydroxybutyric acid | 0.59 ± 0.12 | 0.11 ± 0.02 | 0.19 ± 0.05 |
| Butyryl-CoA | 0.48 ± 0.12 | 1.16 ± 0.26 | 2.42 ± 0.81 |
| Propionyl-CoA | 0.29 ± 0.07 | 0.49 ± 0.08 | 1.69 ± 0.49 |
| 3-Hydroxybutyryl-CoA | 0.13 ± 0.03 | 0.073 ± 0.013 | 0.56 ± 0.14 |
| Acetyl-CoA | 0.64 ± 0.14 | 0.40 ± 0.07 | 0.62 ± 0.16 |
| Succinyl-CoA | 0.37 ± 0.07 | 0.17 ± 0.03 | 0.46 ± 0.12 |