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. Author manuscript; available in PMC: 2010 May 26.
Published in final edited form as: Biochemistry. 2009 May 26;48(20):4305–4313. doi: 10.1021/bi9003759

Table 1.

Enzymatic reactions and assays used for determination of the effects of CT domain mutations in pyruvate carboxylase from R. etli a

Reactionsb Assay
Pyruvate Carboxylation: Forward reaction (BC and CT)c
graphic file with name nihms108684t1.jpg malate dehydrogenase
Oxaloacetate decarboxylation: Reverse reaction (CT and BC domains)
graphic file with name nihms108684t2.jpg hexokinase/glucose-6-
phosphate dehydrogenase
Oxamate-induced oxaloacetate decarboxylation: Reverse reaction (CT)
graphic file with name nihms108684t3.jpg lactate dehydrogenase
Bicarbonate-dependent ATPase: Forward reaction (BC)
graphic file with name nihms108684t4.jpg glucose-6-phosphate
dehydrogenase/
phosphoglucomutase/
phosphorylase a
ADP phosphorylation: Reverse reaction (BC)
graphic file with name nihms108684t5.jpg hexokinase/glucose-6-
phosphate dehydrogenase
a

Products highlighted are those assayed to determine specific activities and initial rates.

b

Detailed reaction and assay conditions in are described in the Methods section.

c

Domains involved in the reaction, BC = biotin carboxylase, CT = carboxyl transferase.