Table 2.

Activities for Pyruvate Carboxylation and Oxaloacetate Decarboxylation

	Pyruvate Carboxylationa			Full Reverse Reactionb
	Vmax (μmol/mg min)	(V/K)c (mM−1 min−1)	% of wt rate	nmol/mg mind	% of wt rate
wild-type	3.5 ± 0.2	7.1 ± 0.9	(100)	5.9 ± 0.2	(100)
K1119Q	NAe	NA	NA	NA	NA
K718Q	0.10 ± 0.08	0.15 ± 0.02	2.9	0.84 ± 0.04	14
T882A	NA	NA	NA	NA	NA
T882S	0.75 ± 0.03	1.0 ± 0.2	21	3.06 ± 0.07	51
T882C	0.25 ± 0.04	0.18 ±0.03	7.1	1.2 ± 0.2	20
Q844L/S885A	0.47 ± 0.07	0.42 ± 0.08	13	3.12 ± 0.08	53

^aReaction conditions: 100 mM Tricine (pH 7.5), 25 °C, 8 mM pyruvate, 10 mM HCO₃ ⁻, 2.5 mM MgATP, 2.5 mM MgCl₂, 0.25 mM acetyl-CoA.

^bReaction conditions: 100 mM Tricine (pH 7.5), 25 °C, 2 mM MgCl₂, 2 mM MgADP, 2 mM phosphate, 0.5 mM oxaloacetate, 0.25 mM acetyl-CoA.

^cPyruvate as variable substrate.

^dNumber of determinations for specific activities = 3.

^eNA = No activity detected.