Table 3.

Oxaloacetate Decarboxylating Activities in the Presence of Oxamate^a

	nmol/mg minb	% of wt rate
wild-type	29 ± 4	(100)
K1119Q	NAc	NA
K718Q	2.1 ± 0.1	7.2
T882A	NA	NA
T882S	8.7 ± 0.3	30
T882C	3.3 ± 0.1	11
Q844L/S885A	1.37 ± 0.3	4.7

^aReaction conditions: 100 mM Tricine (pH 7.5), 25 °C, 0.25 mM acetyl-CoA, 3 mM MgCl₂, 0.5 mM oxaloacetate, 0.5 mM oxamate.

^bNumber of determinations = 3.

^cNA = No activity.