Table 4.

ADP Phosphorylation Activities Using Carbamoyl Phosphate in the Presence and Absence of Free Biotin or Oxamate^a

	nmol/mg minb	% of wt rate	Biotin (10 mM) nmol/mg min	% of wt rate	Oxamate (20 mM) nmol/mg min	% of wt Rate
wild-type	17.8 ± 0.5	(100)	22.5 ± 0.4	(100)	32.4 ± 0.4	(100)
K1119Q	0.184 ± 0.03	1.03	18.2 ± 0.6	81	0.19 ± 0.04	0.57
K718Q	9.3 ± 0.2	52	3.6 ± 0.1	16	10.25 ± 0.06	31
T882A	133 ± 3	747	317.2 ± 0.3	1408	107 ± 5	303
T882S	12.6 ± 0.2	71	43.3 ± 0.1	192	9.6 ± 0.2	30
T882C	5.1 ± 0.1	29	11.2 ± 0.3	49	4.8 ± 0.3	15
Q844L/S885A	5.7 ± 0.4	32	14.4 ± 0.2	64	18.6 ± 0.4	57

^aReaction conditions: 100 mM Tricine (pH 7.5), 25 °C, 2 mM MgADP, 10 mM carbamoyl phosphate, 2 mM MgCl₂, 0.25 mM acetyl-CoA.

^bNumber of determinations = 3.