Table 5.

Bicarbonate-Dependent ATPase Activities in the Presence and Absence of Free Biotin or Oxamate^a

	nmol/mg minb	% of wt rate	Biotin (10 mM) nmol/mg min	% of wt rate	Oxamate (20 mM) nmol/mg min	% of wt rate
wild-type	2.02 ± 0.01	(100)	5.21 ± 0.04	(100)	12.8 ± 0.04	(100)
K1119Q	0.32 ± 0.05	15	4.85 ± 0.07	92	0.33 ± 0.06	1.7
K718Q	1.76 ± 0.04	87	1.32 ± 0.01	25	7.89 ± 0.09	52
T882A	7.20 ± 0.08	356	25.2 ± 0.2	483	2.34 ± 0.05	9.5
T882S	4.5 ± 0.1	222	10.3 ± 0.1	197	3.79 ± 0.02	18
T882C	4.3 ± 0.2	212	8.31 ± 0.04	160	3.32 ± 0.07	19
Q844L/S885A	1.04 ± 0.02	51	3.75 ± 0.02	72	9.87 ± 0.2	58

^aReaction conditions: 100 mM Tricine (pH 7.5), 25 °C, 10 mM HCO₃ ⁻, 2.5 mM MgATP, 2.5 mM MgCl₂, 0.25 mM acetyl-CoA

^bNumber of determinations = 3.