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Inhibition parameters for oxamate and pyruvate using the T882A mutant for the bicarbonate-dependent ATPase reaction
| Vmax (nmol/mg min) | KiNUM (mM) | KiDEN (mM) | KiDEN/KiNUM | |
|---|---|---|---|---|
| Pyruvate a | 7.8 ± 0.3 | 4.9 ± 0.1 | 0.8 ± 0.1 | 0.16 ± 0.02 |
| Oxamate b | 6.8 ± 0.2 | 3.4 ± 0.9 | 1.0 ± 0.2 | 0.29 ± 0.02 |
Reaction conditions: 100 mM Tricine (pH 7.5), 25 °C, 10 mM HCO3 −, 2.5 mM MgATP, 2.5 mM MgCl2, 0.25 mM acetyl-CoA.
0–20 mM pyruvate
0–20 mM pyruvate. Data fitted to eqn 3.
