Table 2.

Inactivation many of the 23 genes by RNAi during adulthood result in increased stress resistance and decreased fecundity

		Stress resistance
Gene	Brief description	Heat*	Juglone†	Reduced fecundity‡
Y39G10AR.8	eIF-2γ	Yes	Yes	Yes
D2085.3	eIF-2Bε	No	No	Yes
D2013.7	eIF-3f	Yes	Yes	Yes
C09D4.5	rpl-19	Yes	Yes	Yes
W01B11.3	Ribosome biogenesis protein Nop58p/Nop5p	Yes	No	Yes
F59C6.4	3′–5′ exoribonuclease subunit involved in rRNA processing	Yes	No	Yes
Y48G1A.4	Nucleolar protein involved in 40S ribosome biogenesis	Yes	Yes	Yes
C47D12.6	Threonyl-tRNA synthetase	Yes	No	Yes
F22D6.3	Asparaginyl-tRNA synthetase	Yes	No	Yes
C48E7.2	RNA polymerase III subunit	Yes	Yes	No
F14B4.3	RNA polymerase I subunit	No	No	Yes
R53.4	Mitochondrial F1F0-ATP synthase subunit f	Yes	Yes	Yes
F59C6.5	NADH:ubiquinone oxidoreductase subunit NDUFB10/PDSW	Yes	Yes	Yes
ZK973.10	NADH:ubiquinone oxidoreductase subunit NDUFS4	Yes	No	Yes
C01F1.2	Cytochrome C oxidase assembly protein	Yes	Yes	Yes
K01C8.6	Mitochondrial rpl-10	Yes	Yes	Yes
F59A3.3	Mitochondrial rpl-24	No	No	Yes
B0511.8	Mitochondrial rps-30	Yes	Yes	Yes
Y47G6A.10	AAA+-type ATPase	Yes	Yes	Yes
F26E4.4	Cell death regulator Aven	Yes	No	Yes
C56E6.1	ABC superfamily transporter	No	No	Yes
K11B4.1	Uncharacterized conserved protein	Yes	No	Yes
B0491.5	Uncharacterized protein	Yes	Yes	Yes

^*Two-day-old adults treated with various RNAi were incubated at 35 °C and their survival was measured. Significant heat stress resistance (P < 0.05, log-rank test) is highlighted.

^†Two-day-old adults treated with different RNAi were incubated on nematode growth medium (NGM) plates containing juglone (360 mm) and their survival were measured. Significant oxidative stress resistance (P < 0.05, log-rank test) was highlighted.

^‡Brood sizes of animals treated with RNAi were measured at 20 °C. P values were calculated using the t-test. Significant brood size decreases (P < 0.05) were highlighted.