Fig. 4.

Overexpression of p8 leads to cell-cycle exit with suppression of myogenic markers. (A,B) C2C12 myoblasts were transfected with control EGFP plasmid or FLAG-tagged human p8, and BrdU incorporation as well as the expression of myogenic and cell-cycle markers determined by immunofluorescence analysis (A). In the topmost panel, transfected cells (EGFP or hp8-FLAG) are red (Alexa Fluor 594), and BrdU+ nuclei are green. In all other panels, transfected cells are green and the endogenous markers (MyoD, p27, myogenin and p21) are red. At least 250 transfected cells were counted in each of two independent experiments and the results quantified B.