Fig. 7.

p8 regulates the association of p68, p300 and MyoD with the myogenin promoter. Chromatin immunoprecipitation analysis of myogenin-promoter occupancy in control and knockdown myoblasts 24 hours after shift to differentiation medium (mean ± s.d., n=4; P-values: ^*0.05; ^**0.005). All four proteins (p8, p68, p300, MyoD) are substantially depleted from the myogenin promoter in p8-knockdown myoblasts, whereas H3 association is unchanged. Although loss of p8 association reflects the specific knockdown of p8 protein, expression levels of p300, p68 and MyoD are unaltered (see Fig. 6D and Fig. 3D, respectively).