Figure 6.—
Chromosome binding of SUUR669–962 upon ectopic expression and its effects on chromatin structure. (A) Ectopic expression from early development in AB1-GAL4>UAS-SuUR669–962 larvae results in the formation of ectopic fibers. (B) Polytene chromosomes from Sgs3-GAL4>UAS-SuUR669-962 larvae resemble wild type in that they show no foam or swellings. (C and E) In Sgs3-GAL4>UAS-SuUR669-962 larvae, the chromosomes are nonspecifically covered with the ectopically expressed truncated protein, as detected with anti-HA antibodies. (D and F) Endogenous SUUR in Sgs3-GAL4>UAS-SuUR669–962 larvae can be detected at its typical chromosomal sites with E45 antibodies. (A–D) Phase contrast. (E and F) Immunostaining.