TABLE 3.

Conservation of charge for middle part

	Negative charge region			Positive charge region
Species	Position	Identity	Charge	Position	Identity	Charge
mel	280–355		−17	356–581		+39
sim	280–350	86.8	−16	351–573	85.3	+37
sec	280–350	85.5	−16	351–576	84.0	+35
yak	280–356	80.5	−18	357–583	78.0	+35
ere	280–356	79.2	−18	357–580	75.8	+41
ana	280–351	56.5	−13	352–580	39.6	+29
pse	280–359	46.9	−18	360–609	40.9	+37
per	280–359	46.3	−18	360–612	40.0	+36
vir	280–371	30.4	−16	372–659	24.3	+43
moj	280–369	26.9	−17	370–676	24.8	+43
gri	280–371	31.5	−16	372–664	21.4	+44

Amino acid identity was calculated between D. melanogaster and other species. mel, D. melanogaster; sim, D. simulans; sec, D. sechellia; yak, D. yakuba; ere, D. erecta; ana, D. ananassae; pse, D. pseudoobscura; per, D. persimilis; vir, D. virilis; moj, D. mojavensis; gri, D. grimshawi.