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Generation and characterization of TS inteins. (A) Principle of TS-intein function. (B) Flowchart of steps used to generate the second-generation TS inteins and to characterize the TS-intein mutations from both the first and second generation. See text for details. Numbers on the right represent the number of TS alleles retained at each step. An asterisk indicates the number of first-generation TS-intein alleles retained at each step.
