Figure 3.—

Growth profiles of yeast FY761 transformed with different pU-Gal4 intein alleles. From top to bottom, intein alleles are listed in order of increasing permissive temperatures, except for controls at the very bottom. Note that the yeast at the bottom grew better than those at the top. Although the transition from one allele to the next is gradual, for the convenience of description, we grouped the TS-intein alleles into five groups. Each row in a panel represents a 1:8 dilution series for the specified intein allele. SD-Ura: synthetic media lacking uracil, dextrose as sugar source; SG-Ura: synthetic media lacking uracil, galactose as sugar source; WT: GAL4 with wild-type intein inserted; Dd: GAL4 with dead intein inserted; F1 to F19 are first-generation TS-inteins; S1 to S88 are second-generation TS-inteins; three-digit numbers in all figures: ID numbers for the TS inteins.