Figure 1.—

Deletion of the histone deacetylase gene RPD3 suppresses the growth defect of esa1. Deletion of RPD3 suppressed the temperature sensitivity of the esa1-414 allele, whereas deletion of other genes encoding histone deacetylases did not. Top panel: serial dilutions of wild type (LPY5), esa1 (LPY4774), esa1 rpd3 (LPY12156), rpd3 (LPY12154), esa1 sir2 (LPY11160), and sir2 (LPY11) are shown on SC at the restrictive temperature for esa1 (35°), compared to growth at the permissive temperature (30°). Bottom panel: serial dilutions of wild type (LPY5), esa1 (LPY4774), esa1 hos1 (LPY13712), esa1 hos2 (LPY13585), esa1 hda1 (LPY13478), hos1 (LPY13706), hos2 (LPY13583), and hda1 (LPY13472) on SC at restrictive and permissive temperatures. Note that some of these interactions overlap with published results from a genomewide study (Lin et al. 2008), yet others are distinct. The differences may be due to strain background- or allele-specific effects (Y. Lin and J. Boeke, personal communication).