Figure 2.—

Rpd3L is the Rpd3-containing complex responsible for suppression of the growth defect in the esa1 mutant. (A) Cartoon highlighting the unique and shared members of the Rpd3S and Rpd3L complexes. (B) Deletion of RCO1, specific to Rpd3S, did not suppress esa1's growth defect at restrictive temperature. Deletion of PHO23 and SDS3, both specific to Rpd3L, mimicked the suppression seen in esa1 rpd3. Serial dilutions of wild type (LPY5), esa1 (LPY4774), esa1 rco1 (LPY12652), rco1 (LPY12645), esa1 sds3 (LPY12956), sds3 (LPY12958), esa1 pho23 (LPY12729), and pho23 (LPY12732), were plated on SC at permissive (30°) and restrictive temperatures (35°). Cartoon of complexes is modified from Roguev and Krogan (2007).