Figure 5.—

Ptc7_s localizes to the mitochondria. In an experiment similar to the one described in Figure 4, we used fluorescent microscopy to establish the cellular localization of Ptc7_s in S. cerevisiae. Three galactose-induced fluorescently labeled variants of Ptc7 were visualized in cells costained with MitoTracker red CMXRos, a fluorescent stain activated upon mitochondrial uptake (Invitrogen). In C, F, and I, the data for Ptc7 and MitoTracker are overlaid, Ptc7 is green, and MitoTracker is red. (A–C) Fluorescent data for yeast expressing only Ptc7_s: (A) GFP, (B) MitoTracker, and (C) GFP and MitoTracker data overlaid. (D–F) Fluorescent data for yeast expressing wild-type Ptc7: (D) GFP, (E) MitoTracker, and (F) GFP and MitoTracker data overlaid. (G–I) Fluorescent data for yeast expressing only Ptc7_u: (G) Venus, (H) MitoTracker, and (I) Venus and MitoTracker data overlaid.