Figure 8.—

The mitochondrial genome affects its own stability. Petite frequencies are shown of isonuclear strains with different mitochondrial genomes constructed via kar crosses (see materials and methods). nDNA, nuclear DNA; mtDNA, mitochondrial DNA. (A) Pooled values from two independent isolates containing BY nDNA and BY mtDNA (UCC8334 and UCC8335) or BY nDNA and RM mtDNA (UCC8338 and UCC8339). (B) The same as in A except strains contain RM nDNA and BY mtDNA (UCC8340 and UCC8341) or RM nDNA and RM mtDNA (UCC8344). (C) BY or RM mtDNA was cytoduced by kar cross (see materials and methods) into UCC8363, which is a BY background strain that contains the MKT1-30G, SAL1, CAT5-91M, and MIP1-661T alleles (referred to as BY 4QTG-RM). Shown are pooled values from two independent isolates containing BY 4QTG-RM nDNA and BY mtDNA (UCC8376 and UCC8378) or BY 4QTG-RM nDNA and RM mtDNA (UCC8372 and UCC8374).