TABLE 4.
Analysis of mitochondrial introns
| PCR confirmation
|
|||
|---|---|---|---|
| Intron | Gene | BY | RM |
| aI1 | COX1 | + | − |
| aI5a | COX1 | + | − |
| aI5b | COX1 | + | − |
| aI5c | COX1 | + | − |
| r1 | 21S_rRNA | + | − |
Total DNA (nuclear and mitochondrial) from either the BY4716 or the RM11-1a strain was used as template in PCR reactions. Primer pairs (see Table S1) annealing to the exons that flank the listed introns were used. A minus (−) indicates that the PCR reaction amplified the shorter product, consistent with the absence of the respective intron in RM. A plus (+) indicates that the presence of the respective intron in BY was confirmed by PCR (see Table S1 for primers). Intron names are according to Pel and Grivell (1993).