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. 2009 Sep;183(1):39–49. doi: 10.1534/genetics.109.104760

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Copyright © 2009 by the Genetics Society of America

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Figure 5.— — Pull-down assays for binding of His₆-DnaA derivatives to BBD-DiaA to stepavidin beads. Samples were analyzed by Western blotting with Anti-His₆ antibody. (Lane A) Crude lysate BBD-DiaA alone. (Lane B) Crude lysate of His₆-DnaA⁺ before pulldown. (Lane C) Crude lysate His₆-DnaA_Δ96-120 before pulldown. (Lane D) BBD-DiaA-streptavidin beads with no His-tagged DnaA added. (Lane E) BBD-DiaA-streptavidin beads with bound His₆-DnaA⁺. (Lane F) Protein standard. (Lane G) BBD-DiaA-streptavidin beads with bound His₆-DnaA_Δ96-120. (Lane H) His6-DnaA⁺ added to streptavidin beads with no BBD-DiaA bound.