Figure 1.—

Sequence comparison of KlAft, Aft1, and Aft2. The amino acid sequences of KlAft, Aft1, and Aft2 were aligned using the Clustalw program (Larkin et al. 2007). This alignment was then manually refined with the Seaview program (Galtier et al. 1996). The NES-like sequence of Aft1 (Yamaguchi-Iwai et al. 2002) and the homologous regions in Aft2 and KlAft are boxed. The conserved leucines critical for nuclear export of Aft1 are in boldface type. Conserved cysteine and histidine residues predicted to participate in a Zn-finger domain (Babu et al. 2006) are in boldface type. Conserved cysteines involved in the CDC element are in boldface type and this element is boxed. Glutamine-rich regions were detected with the ScanProsite tool (De Castro et al. 2006).