Figure 4.

Transcription factors inhibited by PRL treatment in decidua and ovary of PRLR-/-RS mice. Day 9 pseudopregnant PRLR-/-RS mice were injected with ergocryptine (200 μg, sc) for 6 hr followed by a single ip injection of either PRL (60 ug) or vehicle (control). Decidua and ovaries were obtained after 0, 30 and 120 min of PRL treatment. DNA binding activities of transcription factors were measured by Protein/DNA binding assay using nuclear extracts from decidua. (A) Transcription factors whose DNA binding activities are inhibited at both time points (upper panel) or inhibited only at 30 min (lower panel) by PRL treatment in decidua. (B) Transcription factors whose DNA binding activities are inhibited at 120 (upper panel) or 30 min (lower panel) by PRL treatment in ovary.