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Semi-quantitative RT-PCR analysis of sHsps expression under heat, cold, air drying and osmotic stress. Total RNA from the roots and shoots of 7 d old seedlings, dry seeds and panicle was used for RT-PCR analysis. Heat stress (HT): 0.5 h at various temperatures; Osmotic stress: 15% PEG 4000; Dehydration stress: air drying (AD) 20 min; low temperature (LT): 6°C ± 2. Ubq5 was used as internal control for RT reaction.
