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1H-15N and 11H-13C HSQC correlation spectra at 10°C for TAR RNA uniformly labeled with 15N-labeled guanosine and adenosine labeled with 13C at positions 2 and 8. (a) Sequence of the partially labeled TAR-2 construct. (b) One bond correlations between protons and the labeled carbons in the four adenosine bases. (c) One bond correlations between N1 the eight hydrogen-bonded imino protons. (d) Two bond correlations between the non-exchangeable H8 proton and the N7 atom.
