Table 1.

Complete list of enzymes for de novo enzymatic synthesis of ATP and GTP. The amounts of each enzyme used for synthesis of compounds 1–4 in Figure 4 is provided as either Units of enzyme or mg of enzyme.

Enzyme	Gene	EC	Source	1	2	3	4
Hexokinase	hxk1/2	2.7.1.1	BY	5.6 U	5.6 U	---	---
Glucokinase	glk	2.7.1.2	RE	---	---	2.4 U	2.4 U
Glucose-6-phosphate dehydrogenase	zwf1	1.1.1.49	BY	6.2 U	6.35 U	---	---
Glucose-6-phosphate dehydrogenase	zwf	1.1.1.49	RE	---	---	2.6 U	2.6 U
Phosphogluconate dehydrogenase	gndA	1.1.1.44	RE	6.8 U	6.85 U	2.9 U	2.9 U
Ribose-5-phosphate isomerase	rpiA	5.3.1.6	RE	7.5 U	7.5 U	3.2 U	3.2 U
Ribose-phosphate diphosphate kinase	prsA	2.7.6.1	RE	8.2 U	8.2 U	3.5 U	3.5 U
Amidophosphoribosyl-transferase	purF	2.4.2.14	RE	10.5 mg	15.2 mg	4.0 mg	4.0 mg
Phosphoribosylamine-glycine ligase	purD	6.3.4.13	RE	14.4 mg	12.5 mg	4.3 mg	4.3 mg
Phosphoribosylglycinamide formyltransferase	purN	2.1.2.2	RE	4.6 mg	7.5 mg	4.7 mg	4.7 mg
Phosphoribosylformylglycinamidine synthase	purL	6.3.5.3	RE	25.6 mg	25.2 mg	5.4 mg	5.4 mg
Phosphoribosylformylglycinamidine cyclo-ligase	purM	6.3.3.1	RE	11.7 mg	7.8 mg	5.7 mg	5.7 mg
Phosphoribosylamino-imidazole carboxylase (catalytic subunit)	purE	4.1.1.21	RE	18.7 mg	35.4 mg	6.5 mg	6.5 mg
Phosphoribosylamino-imidazole carboxylase (ATPase subunit)	purK	4.1.1.21	RE	6.0 mg	8.5 mg	6.2 mg	6.2 mg
Phosphoribosylamino-imidazole-succinocarboxamide synthase	purC	6.3.2.6	RE	3.4 mg	6.9 mg	7.0 mg	7.0 mg
Adenylosuccinate lyase	purB	4.3.2.2	RE	56 U	28 U	10 U	10 U
Phosphoribosylamino-imidazole-carboxamide formyltransferase	purH	2.1.2.3	RE	14 mg	31 mg	18 mg	18 mg
Inosine-monophosphate cyclohydrolase	purH	3.5.4.10	RE
Adenylosuccinate synthase	purA	6.3.4.4	RE	10 U	---	---	---
Inosine-monophosphate dehydrogenase	guaB	1.1.1.205	RE	---	25.4 U	15 U	15 U
Guanosine-monophosphate synthase	guaA	6.3.5.2	RE	---	25.8 U	16.5 U	16.5 U
Adenylate kinase	ak5	2.7.4.3	CM	200 U	---	---	---
Adenylate kinase	plsA	2.7.4.3	RE	---	47 U	9 U	9 U
Creatine phosphokinase	ckmT	2.7.3.2	RM	250 U	118 U	---	---
Creatine phosphokinase	ckmT	2.7.3.2	RE	---	---	125 U	125 U
Guanylate kinase	spoR	2.7.4.8	RE	1 U	55 U	18 U	18 U
Glycine hydroxymethyltransferase	glyA	2.1.2.1	RE	22.5 mg	25 mg	20.2 mg	20.2 mg
Methylene-tetrahydrofolate dehydrogenase	folD	1.5.1.5	RE	10.5 mg	23.4 mg	20.2 mg	20.2 mg
Methenyl-tetrahydrofolate cyclohydrolase	folD	3.5.4.9	RE
Aspartate ammonia-lyase	aspA	4.3.1.1	RE	92 U	32 U	20 U	20 U
Glutamate dehydrogenase	glud1	1.4.1.3	BL	94 U	141 U	---	---
Glutamate dehydrogenase	gdhA	1.4.1.4	RE	---	---	39 U	39 U
Glutamine synthase	glnA	6.3.1.2	RE	---	---	38 U	---
Inorganic diphosphatase	ppa	3.6.1.1	RE	23.4 U	24 U	26 U	26 U

Source Key: RE = Recombinant from E. coli, BY = Baker’s yeast, CM = Chicken muscle, RM = Rabbit muscle, BL = Bovine liver