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. 2009 Sep 18;15:1897–1905.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The experimental scheme of topical VS application for laser-induced CNV. A: Therapeutic efficacy was assessed by flat mount analysis. CNV was induced by laser photocoagulation on day 0 and validated on day 21 by FAG analysis. The animals (n=8) were euthanized on day 24 to measure CNV extent by flat mount analysis. B: In serial FAG analysis, CNV was induced by laser photocoagulation at day 0. From day 1 to 20 after induction, the animals were either left untreated (CNV control group, n=8) or treated with VS eye drops (1 μg/ml in PBS; CNV+VS group, n=8), or PBS vehicle (CNV+PBS group; n=8) three times per day.