ELISA reactivities of sera from patients with sporotrichosis (Sporo) and from normal human subjects (NHS) diluted 1:100, with Sepia and synthetic melanins and with the following eight different types of S. schenckii melanin-particles (50 μg/well): mycelium grown in minimal medium (MM-M), mycelium grown on minimal medium with tricyclazole (MMT-M), mycelium grown on minimal medium with L-DOPA (MML-M), mycelium grown on minimal medium with tricyclazole and L-DOPA (MMLT-M), yeast grown on minimal medium (MM-Y), yeast grown on minimal medium with tricyclazole (MMT-Y), yeast grown on minimal medium with L-DOPA (MML-Y) and yeast grown on minimal medium with L-DOPA and tricyclazole (MMLT-Y). OD, optical density. * P < 0.0001; # P < 0.05; ns: not significant.