Table 2. Myogenic Induction Media for MSC.
| Origin of MSC | Media | Serum | Supplementation |
|---|---|---|---|
| Adipose tissue (120) | DMEM | 10% FBS, 5% HS | 50 μM hydrocortisone, 1% anitibiotic/antimycotic |
| Bone marrow (121) | DMEM | 10% FBS, 5% HS | 10 μmol/L 5-azacytidine |
| Amniotic membrane (77) | DMEM | 5% FBS, 40% MCDB-201; ITS-LA BSA 100X | 10−8M dexamethasone, 10−4M ascorbic-acid-2- phosphate, 10 ng/ml bFGF, 10 ng/ml VEGF, 10 ng/ml IGF-1 |
| Embryonic stem cells (122) | Alpha MEM | 3% HS, 1% FBS | Co-culture with C2C12 cells |
| Fetal blood (119) | Myoblast- conditioned media | None | None |
| Fetal blood (123) | Galectin- enriched media* | None | *100–1,000 ng/ml galectin-1 |
| Adipose tissue (124) | DMEM high glucose | 20% FBS | Co-culture with primary myoblasts, 2 mM L- glutamine, 1% penicillin-streptomycin |
| Umbilical cord blood (125, 126) | DMEM | 10% FBS, 5% HS, | 0.1μM dexamethasone, 50 μM hydrocortiosne |
| Fetal aorta (127) | EBM-GM | None | 50 ng/ml platelet-derived growth factor (PDGF)-ββ |
| Bone marrow (128) | DMEM | 2% HS | Wnt3a conditioned media |
| 10T1/2 cells (129) | DMEM | 10% FBS | 20 μM 5-azacytidine, 0–300 nM testosterone |
DMEM: Dulbecco’s modified Eagle’s medium; FBS: Fetal bovine serum; HS: horse serum; MCDB-201: chick fibroblast basal medium; ITS-LA BSA: insulin, transferrin, selenic acid-linoleic acid-bovine serum albumin; VEGF: vascular endothelial growth factor; IGF-1: insulin-like growth factor; bFGF: basic fibroblast growth factor; EBM-GM: endothelial basal medium growth medium.