Table 1.

Antibodies and their concentrations, manufacturers, and application for western blot

Primary antibodies	S	Dilutions	Manufacturer	Cat#
P-Akt (Ser473)	Rab	1 : 1000	Cell signaling,  MA, USA	9271
Akt	Rab	1 : 1000	Cell signaling	9272
P-PTEN (Ser380)	Rab	1 : 1000	Cell signaling	9551
PTEN	Rab	1 : 1000	Cell signaling	9552
P-PDK1 (Ser241)	Rab	1 : 1000	Cell signaling	3061
PDK1	Rab	1 : 1000	Cell signaling	3062
P-GSK3b (Ser 9)	Rab	1 : 1000	Cell signaling	9336
GSK3b	Rab	1 : 1000	Cell signaling	9332
P-β-catenin	Rab	1 : 1000	Cell signaling	9561
β-catenin	Ms	1 : 1000	Upstate  Biotechnology,  NY, USA	05-482
Active β-catenin	Ms	1 : 1000	Calbiochem,  CA, USA	219357  (7D11)
P-JNK	Rab	1 : 1000	Cell signaling	9251
JNK	Rab	1 : 1000	Cell signaling	9252
P-ERK1/2	Rab	1 : 1000	Cell signaling	9101
ERK1/2	Rab	1 : 1000	Cell signaling	9102
P-δPKC (Thr505)	Rab	1 : 1000	Cell signaling	9374
δ-PKC	Rab	1 : 5000	Santa Cruz  Biotechnology,  CA, USA	sc-213
P-εPKC	Rab	1 : 1000	Upstate  Biotechnology	06-821
ε-PKC	Rab	1 : 10 000	Santa Cruz  Biotechnology	sc-214
β-actin	Ms	1 : 20 000	Sigma, MO, USA	A-3854
β-actin	Rab	1 : 20 000	Bethyl, TX, USA	A300-491A

PKC, protein kinase C; P-εPKC, phosphorylated P-εPKC; P-δPKC, phosphorylated P-δPKC; ERK extracellular signal-regulated kinase; P-ERK, phosphorylated ERK; JNK, c-jun N-terminal kinase; P-JNK, phosphorylated-JNK; GSK3β, glycogen synthase kinase; P-GSK3β, phosphorylated GSK3β; PDK1, phosphoinositide-dependent protein kinase-1; P-PDK1, phosphorylated PDK1; PTEN, phosphatase and tensin homologue deleted on chromosome 10; P-PTEN, phosphorylated PTEN; P-β-catenin, phosphorylated β-catenin S, species; Rab, rabbit; Ms, Mouse.