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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Cell Cycle. 2009 Apr 23;8(8):1256–1270. doi: 10.4161/cc.8.8.8287

Figure 2.

Figure 2

Chronological aging is caused by a cell-extrinsic factor. (A) BY4743, (B) W303AR5 or (C) DBY476 were grown in SC 2% or SC 0.05% medium for 2 days then resuspended in supernatant from isogenic cells grown in either in SC 2% or SC 0.05% medium for 2 days. In each case, cells grown for 2 days in SC 2% then transferred to cell free pre-conditioned medium from 2 day old SC 0.05% yeast (SC 2% → SC 0.05%) lived significantly longer than cells maintained in SC 2% medium (SC 2%). Cells grown for 2 days in SC 0.05% then transferred to cell free pre-conditioned medium from 2 day old SC 2% yeast (SC 0.05% → SC 2%) lived significantly shorter than cells maintained in SC 0.05% medium. The asterisk in (C) indicates culture re-growth, a phenomenon known as gasping that is observed in chronologically aging cultures when a small fraction of the population re-enters the cell cycle. Error bars are standard deviation of three biological replicates.