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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Cell Cycle. 2009 Apr 23;8(8):1256–1270. doi: 10.4161/cc.8.8.8287

Figure 5.

Figure 5

Acetic acid is specifically toxic to yeast cells. (A) BY4743 cells were aged in SC 2%, SC 0.05%, or pre-grown for 48 hours in SC 0.05% then buffered to pH 2.6 with the addition of a concentrated buffer (see Materials and Methods). Arrow indicates age at buffer addition. Error bars indicate the standard deviation of three biological replicates. (B) Acetic acid significantly reduced survival of 4 day old yeast cells cultured in SC 2%, but neither malic nor citric acid had a similar effect, even though the pH was similar in each case (Table S1). (C) Addition of hydrochloric acid at a concentration sufficient to decrease pH to a level comparable to 500 mM acetic acid did not reduce survival of 4 day old yeast cells. (D) Only acetic acid (pH 2.5) and not the conjugate base (pH 6.0) caused cell death in BY4742 cells. Error bars indicate standard deviation of three technical replicates.