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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Cell Cycle. 2009 Apr 23;8(8):1256–1270. doi: 10.4161/cc.8.8.8287

Figure 6.

Figure 6

Acetic acid is sufficient to cause chronological aging. (A) 2-day old BY4743 cultures were transferred to water adjusted to pH 2.8 with HCl and maintained at a concentration of 10 mM acetic acid. Acetic acid concentration was monitored every 1–2 hrs for the first 36 hours and additional acetic acid was provided as needed to maintain a concentration of 10 mM (see Suppl. Fig. S8A). The total molar amount of acetic acid added was 108.6 mM. (B) 2-day old BY4743 cultures grown in SC 0.05% were first lowered to pH 2.8 with HCl and then supplemented with 10 mM acetic acid. Acid was monitored similarly as (A) and added as needed over the first 36 hours (see Suppl. Fig. S8B). Mortality curve for SC 0.05% pH 2.8 + acetic acid was normalized for culture growth observed during the course of adding the acid (see Suppl. Fig. S8C) by dividing the viability by the ratio of culture ODs (pH 2.8 + acetic acid/pH 2.8 alone). Error bars indicate the standard deviation of 3 biological replicates for untreated and pH 2.8 cultures, and 6 biological replicates of pH 2.8 cultures maintained at 10 mM acetic acid.