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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Exp Cell Res. 2009 Jan 18;315(6):996–1007. doi: 10.1016/j.yexcr.2009.01.003

Fig. 2.

Fig. 2

All domains of lamin A are required for full repression activity in yeast. (A) Repression of lacZ reporter in yeast by Gal4DBD-fusion proteins of lamin A domains. The graph shows the fold repression of the fusion proteins over GAL4DBD empty vector alone. The data shown is representative of at least three independent transformants. (B) Gal4DBD-intermediate filament fusion proteins were expressed from ARS/CEN plasmids under ADH1 promoter. Results shown are fold repression over GAL4DBD empty vector alone (n=3, mean±SD). Western blot analysis was performed to verify the expression of fusion proteins. Protein samples were separated by using SDS-4-20% PAGE and detected by immunoblotting with mouse anti-Gal4DBD. The relative protein levels were determined by counting pixel levels using NIH ImageJ and represented as a percentage of total pixels counted. (C) Repression of lacZ reporter in yeast by Gal4DBD-intermediate filament protein C-terminus domains. The graph presents the fold repression of the fusion proteins over GAL4DBD empty vector alone. The data shown are representative of at least three independent transformants. (D) Repression of lacZ reporter in yeast by Gal4DBD-fusion proteins of intermediate filament chimeras. Chimeras were made by interchanging the domains between lamin A and vimentin. Results shown are fold repression over GAL4DBD empty vector alone (n=3, mean±SD).