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. 2009 Jul 14;114(12):2530–2541. doi: 10.1182/blood-2009-04-214403

Figure 3.

Figure 3

Analysis of differentially expressed mobilized CD34+ cells genes by qRT-PCR. The expression of 5 genes (FLT3, HOXA9, PTGS2, CXCR4, and CTSL) in CD34+ cells from the 3 rhesus macaque mobilized by plerixafor (A), G-CSF (G), or plerixafor plus G-CSF (A+G) were analyzed by qRT-PCR. The expression of CXCR4 was greater in plerixafor and plerixafor plus G-CSF–mobilized CD34+ cells. The expression of CTSL was greater on G-CSF– and G-CSF plus plerixafor-mobilized CD34+ cells. FLT3 expression was greatest on plerixafor-mobilized CD34+ cells, and the expression of PTGS2 was greatest on plerixafor plus G-CSF–mobilized cells. The level of expression of HOXA9 by the 3 types of CD34+ cells was similar but much greater than peripheral blood leukocytes. The expression of the 5 genes in leukocytes that were not absorbed by anti-CD34 from the 9 mobilized PBSC concentrates (CD34−) are shown as a control.