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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: Free Radic Biol Med. 2008 Nov 1;46(2):253–262. doi: 10.1016/j.freeradbiomed.2008.10.029

Figure 6.

Figure 6

(A) Effect of melatonin on the reduction of NQO2-FAD by BNAH. The absorption spectrum of 10 U NQO2 was recorded before (a) and after sequential addition of 2.5 eq of melatonin (b), 0.9 eq BNAH (c) and 1.2 eq substrate (d). Control spectrum (e) was obtained for melatonin alone. (B) Inhibition of reduction of NQO2-FAD by BNAH with quercetin. The spectroscopic analysis was carried out as described in Methods. The absorption spectrum of 10 U NQO2 was recorded before (a) and after (b) addition of 0.9 eq BNAH. In the inhibition studies, 10 μM quercetin was added to a cuvette containing 10 U NQO2 prior to the addition of 0.9 eq BNAH (c).